IMAC Agarose High Performance

• Cat.No.: IMAC HP-001A
• Product Overview: IMAC agarose High Performance media is easily charged by the user with the metalion of choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.

Specification

• Tag: Non
• Cat.no: IMAC HP-001A
• Characteristics: 1. For optimizing purification of histidine-tagged proteins when Ni2+ is not the best choice of metal ion2. Conveniently charge with your metal of choice3. 34 μm bead size for high performance purification4. High binding capacity
• Size: 25 mL
• Matrix: Highly cross-linked agarose, 6%
• Ion exchange capacity: Approx. 15 μmol Ni2+/ml medium
• Average particle size: 34μm
• Dynamic binding capacity: At least 40 mg histidine-tagged protein/ml medium when changed with Ni2?
• Recommended flow rate: <300>
• Recommended column height: 5‐20cm
• Chemical stability: Stable in all commonly used buffers, At 40°Cc0.01 M HCl, 0.1 M NaOH [For One Week] 1M NaOH, 70% acetic acid [For 12 h]30% 2-propanol [30 min] 2% SDS [1 h]
• pH working range: 3-12
• pH CIP range: 2-14
• Temperature Stability: 4-30°C
• Warning: Avoid using Chelating agents, e.g. EDTA, EGTA, citrate