||Invertase Assay Kit is a quantitative determination of invertase/sucrase activity.
||INVERTASE (β-fructofuranosidase, EC 220.127.116.11) is an enzyme that catalyzes the hydrolysis of sucrose to fructose and glucose. Invertases cleave at the O-C(fructose) bond, whereas a related enzyme sucrose (EC 18.104.22.168) cleaves at the O-C(glucose) bond. A wide range of microorganisms produce invertase and can, thus, utilize sucrose as a nutrient. Invertase assay finds wide applications in environmental (e.g. soil), agricultural and food (confectionery) industry. Invertase Assay Kit provides a convenient and ultra-sensitive colorimetric and fluorimetric means to measure invertase activity. In the assay, invertase cleaves sucrose, resulting in the formation of fructose and glucose, which is determined by a colorimetric (570nm) or fluorimetric method (λem/ex = 585/530nm). The assay is simple, sensitive, stable and high-throughput adaptable.
||Invertase and sucrase activity determination in biological and environmental (e.g. soil) samples.Evaluation and screening for invertase inhibitors.
||For research use only (RUO)
||Store 10x Reaction Buffer and Assay Buffer at 4°C and other reagents at -20°C. Shelf life of six months after receipt.
||10x Reaction Buffer: (pH 4.5) 12 mLAssay Buffer 10 mLGlucose Standard 1 mL10x Sucrose 1.5 mLEnzyme Mix 120 uLDye Reagent 120 uL
|Compatible Sample Types:
||Biological Sample, Environmental (e.g. soil) Sample
|Features & Benefits:
||Safe: Non-radioactive assay.Sensitive and accurate: As low as 0.007 U/L invertase activity can be quantified.Homogeneous and convenient: "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved.Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day.