Lambda Fab Select Kit

• Cat.No.: Lambda-001A
• Product Overview: Lambda Fab Select is affinity chromatography media for purifying lambda Fab fragments, which enable efficient capture with high purity and yield.

Specification

• Tag: Non
• Cat.no: Lambda-001A
• Form: suspension in 20% ethanol
• Matrix: Highly cross-linked agarose
• Average particle size: 75 μm
• Ligand: Recombinant protein (Mr 13 000), produced in S. cerevisiae, with affinity for the constant domain of the immunoglobulin Fab lambda light chain
• Ligand concentration: Approx. 7 mg/ml of medium
• Dynamic binding capacity: Approximately 20 mg Fab/mL of medium
• Recommended flow rate: At least 600 cm/h in a 1 m diameter column, with 20 cm bed height at 20°C using buffers with the same viscosity as water at< 3="">
• Chemical stability: Stable to all commonly used aqueous buffers: 6 M urea, 6 M guanidine hydrochloride, 70 % ethanol, 30 % isopropanol
• pH working range: 3-10
• pH CIP range: 2-12
• Temperature Stability: 4–30 °C
• Elution buffer: 0.1 M acetate buffer, pH 3.5
• Warning: Prolonged exposure (i.e., several days) to pH< 2="" should="" be="" avoided="" due="" to="" slow="" decomposition="" of="" the="" agarose="" matrix="" at="" low="" ph.="" for="" kappaselect="" avoid="" ph="">12.