| Product Overview : |
Allows fast and easy measurement of lipase activity in vitro, in cell preparations or in vivo using the fluorescent fatty acid substrate 1,2-dioleoyl-3-(pyren-1-yl)decanoyl-rac-glycerol. Lipases are a family of hydrolytic enzymes that release fatty acids from triacylglycerols in a site-specific manner. Most lipases have optimum activity for the primary ester groups of triglycerides. While some lipases remove fatty acyl groups from either the C-1 or C-3 positions, others remove both C-1 and C-3 acyl groups. Only specific lipases will cleave the C-2 acyl group from triacylglycerols. The substrate is typically not a single molecule but a nonaqueous phase of aggregated lipid. Lipase activity, ubiquitous among most cells, can be monitored using the new fluorescent substrate kit that utilizes a fluorescent triglyceride 1,2-dioleoyl-3-pyrenedecanoyl-rac-glycerol. Upon cleavage, the fluorescent fatty acid, pyrenedecanoic acid, is released and activity measurements are easily obtained either in vitro (in cell preparations) or in vivo. The fluorescent triglyceride substrate also will have applications to other research areas including micelle and membrane labeling, fluorescence energy transfer experiments, and membrane fluidity studies. |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.