Lysozyme Inhibitor Screening Kit

• Cat.No.: Kit-2099
• Product Overview: Lysozyme Inhibitor Screening Kit uses N,N′,N′′-Triacetylchitotriose -a competitive inhibitor that binds to the active site of lysozyme. The inhibitor decreases the catalytic ability of lysozyme to hydrolyze a fluorogenic substrate. The kit provides a simple, rapid, sensitive and reliable test suitable for screening of lysozyme inhibitors.

Specification

• Size: 100 assays
• Applications: The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput screening of Lysozyme inhibitors. nhibitor control (N,N,N-Triacetylchitotriose) is included to compare the efficacy of test inhibitors.
• Storage: Store kit at -20°C, protected from light. Briefly centrifuge small vials prior to opening. Read entire protocol before performing the assay.Lysozyme Assay Buffer: Warm to 37°C before use. Store at either 4ºC or -20 ºC.Lysozyme Substrate: Aliquot and store at -20 ºC. Bring to room temperature before use.Lysozyme: Reconstitute with 1060 μl Lysozyme Assay Buffer, pipette up and down to mix thoroughly. Aliquot and store at –20°C. Avoid repeated freeze/thaw. Keep on ice while in use. Stable for 2 months after reconstitution.N,N′,N′′-Triacetylchitotriose: Reconstitute with 60 μl dH2O and store at -20°C. Use within two months.