MabAffinity rProtein A Agarose High Flow

• Cat.No.: Potein A-001A
• Product Overview: Mab Affinity Protein A Agarose High Flow is a high capacity, chemically stableaffinity medium designed specially for the purification of monoclonal and polyclonal antibodies at laboratory and process scales.

Specification

• Tag: Non
• Cat.no: Potein A-001A
• Form: Slurry in 20% Ethanol
• Size: 5 mL, 25 mL, 100 mL
• Matrix: Highly cross-linked Agarose
• Molecular Characterization: Recombinant protein A has a molecular weight of Mr 44 600
• Average particle size: 90 μm
• Ligand: Protein A
• Coupling chemistry: Epoxy
• Dynamic binding capacity: >30mg IgG /ml media
• Recommended flow rate: 100-300cm/h
• Viscosity: Unused media can be stored in the container at a temperature of +4 to +8°C. Ensure that the screw top is fully tightened.Packed columns should be equilibrated in binding buffer containing 20% ethanol to prevent microbial growth.After storage, equilibrate with at least 5 bed volumes of starting buffer before use.
• Recommended column height: 5‐20cm
• Chemical stability: Stable in all aqueous buffers commonly used in protein A chromatography
• pH working range: 3-9
• pH CIP range: 2-11
• CIP stability: 8M Urea, 6M Gua‐HCl or 1mM NaOH+1MNaCl pH 11, 0.1M acetic acid
• Temperature Stability: 2‐40 °C
• Elution buffer: 100 mM glycin, 10 mM NaCl, pH 3.0
• Shelf life: 5 years
• Warning: 1. Determined at 10% breakthrough at 4min residence time2. Delivered at room temperature, and recommended long‐term storage at 2‐8 °C