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MabAffinity rProtein A Agarose High Flow

Cat.No. : Potein A-001A
Product Overview : Mab Affinity Protein A Agarose High Flow is a high capacity, chemically stableaffinity medium designed specially for the purification of monoclonal and polyclonal antibodies at laboratory and process scales.
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Cat.no : Potein A-001A
Form : Slurry in 20% Ethanol
Size : 5 mL, 25 mL, 100 mL
Matrix : Highly cross-linked Agarose
Molecular Characterization : Recombinant protein A has a molecular weight of Mr 44 600
Average particle size : 90 μm
Ligand : Protein A
Coupling chemistry : Epoxy
Dynamic binding capacity : >30mg IgG /ml media
Recommended flow rate : 100-300cm/h
Viscosity : Unused media can be stored in the container at a temperature of +4 to +8°C. Ensure that the screw top is fully tightened.Packed columns should be equilibrated in binding buffer containing 20% ethanol to prevent microbial growth.After storage, equilibrate with at least 5 bed volumes of starting buffer before use.
Recommended column height : 5‐20cm
Chemical stability : Stable in all aqueous buffers commonly used in protein A chromatography
pH working range : 3-9
pH CIP range : 2-11
CIP stability : 8M Urea, 6M Gua‐HCl or 1mM NaOH+1MNaCl pH 11, 0.1M acetic acid
Temperature Stability : 2‐40 °C
Elution buffer : 100 mM glycin, 10 mM NaCl, pH 3.0
Shelf life : 5 years
Warning : 1. Determined at 10% breakthrough at 4min residence time2. Delivered at room temperature, and recommended long‐term storage at 2‐8 °C

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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