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| Cat.No. : | Kit-0533 |
| Product Overview : | Maleimide Assay Kit is a quantitative assay for the determination of maleimide groups. |
| Description : | Maleimides can be directly assayed spectrophotometrically at 302 nm. However, the small extinction coefficient of 620 M-1cm-1 renders this assay insensitive, and the assay is further complicated by the protein absorbance at the same wavelength. |
| Applications : | This colorimetric maleimide assay kit quantifies maleimide groups by first reacting a sample with a known amount of thiol present in excess and then assaying the remaining unreacted thiol using 4,4"-DTDP with a molar extinction coefficient of 19,800 M-1cm-1. |
| Usage : | For research use only (RUO) |
| Storage : | Store the kit at -20°C and avoid from light. |
| Kit Components : | Component A: MEA 1 vialComponent B: 4,4"-DTDP 1 vialComponent C: Assay Buffer 50 mLComponent D: DMSO 1 mL |
| Detection method : | Colorimetric |
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