Maleimide Assay Kit

• Cat.No.: Kit-0533
• Product Overview: Maleimide Assay Kit is a quantitative assay for the determination of maleimide groups.

Specification

• Tag: Non
• Description: Maleimides can be directly assayed spectrophotometrically at 302 nm. However, the small extinction coefficient of 620 M-1cm-1 renders this assay insensitive, and the assay is further complicated by the protein absorbance at the same wavelength.
• Applications: This colorimetric maleimide assay kit quantifies maleimide groups by first reacting a sample with a known amount of thiol present in excess and then assaying the remaining unreacted thiol using 4,4"-DTDP with a molar extinction coefficient of 19,800 M-1cm-1.
• Usage: For research use only (RUO)
• Storage: Store the kit at -20°C and avoid from light.
• Kit Components: Component A: MEA 1 vialComponent B: 4,4"-DTDP 1 vialComponent C: Assay Buffer 50 mLComponent D: DMSO 1 mL
• Detection method: Colorimetric