Methyltransferase Fluorometric Assay Kit

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Methyltransferase Fluorometric Assay Kit

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Cat.No. : Kit-0610
Product Overview : Methyltransferase Fluorometric Assay Kit is a continuous enzyme-coupled assay that can continuously monitor SAM-dependent methyltransferases. The removal of the methyl group from SAM generates S-adenosylhomocysteine (AdoHcy), which is rapidly converted to S-ribosylhomocysteine and adenine by AdoHcy nucleosidase. This rapid conversion prevents the buildup of AdoHcy and its feedback inhibition on the methylation reaction. Finally, the adenine is converted to hypoxanthine, by adenine deaminase, which in turn is converted to urate and hydrogen peroxide (H2O2). The reaction between H2O2 and ADHP (10-acetyl-3,7,-dihydroxyphenoxazine) produces the highly fluorescent compound resorufin. Resorufin fluorescence can be easily analyzed with an excitation wavelength of 530-540 nm and an emission wavelength of 585-595 nm. The assay is supplied with AdoHcy as a positive control. The assay can be used with any purified SAM-dependent methyltransferase.
Storage : Stability; 6 months;Storage: -80°C;This kit will perform as specified if stored as specified in the Materials Supplied section, and used before the expiration date indicated on the outside of the box.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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