Mitochondrial Complex V Activity Assay Kit
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|Product Overview :||The Mitochondrial Complex V (ATP Synthase) Activity Assay Kit is used to determine the activity of this enzyme in a sample. ATP Synthase is immunocaptured within the wells. The enzyme functions by hydrolyzing ATP to ADP and phosphate. This production of ADP is ultimately coupled, as shown below, to the oxidation of NADH to NAD+ which is monitored as a decrease in absorbance at 340 nm (See References section for an article that describes the nature of this assay). The ATP hydrolysis activity and therefore the coupled reaction is inhibited by oligomycin (a specific inhibitor of ATP Synthase).|
|Size :||96 rxn|
|Storage :||Store Buffer, Detergent, and covered Microplate at 2 to 8°C. Store the Phospholipids and Reagent Mix at -80°C. For multiple experiments, it is recommened to aliquot the Reagent Mix and Phospholipids upon initial thaw and store any aliquots at -80°C.|
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