MMP-3 Activity Assay Kit Kit-0588

Cat.No. :	Kit-0588
Product Overview :	The assay sensitivity is< 50 µU. (100 assays) Simple procedure; takes ~ 60 minutes;Fast and convenient;Sensitive assays for measuring MMP-3 in various biological samples.
Size :	100 assays
Description :	The matrix metalloproteinase-3 (MMP-3, stromelysin-1) exhibits a number of activities that would make it a particularly good tumor promoter. Like several other MMPs, MMP-3 was first cloned and later recloned as a cancer-specific gene. In addition to degrading numerous extracellular matrix components, MMP-3 can activate gelatinase B, the collagenases and several serpin-type serine proteinase inhibitors. Moreover, it can release a number of cell surface molecules, including E-cadherin, a known contributor to cancer development. In MMP-3 Assay Kit, MMP-3 hydrolyzes a specific FRET substrate to release the quenched fluorescent group Mca, which can be detected fluorometrically at Ex/Em = 325/393 nm. The kit provides a rapid, simple, sensitive and reliable test which can also be used as a high throughput assay of MMP-3 activity. The assay sensitivity is< 50 µU.
Applications :	The assay sensitivity is< 50 µU.
Target Species :	Mammalian
Usage :	This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.
Kit Components :	MMP-3 Assay BufferMMP-3 SubstrateMca Standard (1mM)MMP-3 Positive Control
Detection method :	Fluorometer
Compatible Sample Types :	Cell Culture SupernatantsPlasmaSerumUrine