N-Glycan Label, Mass Spec Grade

• Cat.No.: N-Glycan Label-53
• Product Overview: Molecular Formula: C21H25N5O5 Mass Shift: + C17H20N4O2 = 312.3663 Da Labeled N-Glycan (Da) = Released N-Glycan + Glycan Label Reagent - Reaction by-product ( C4H5NO3, MW: 115.0268) - H Product Composition: The ratio of N-Glycan Label: NHS compound is 3:2 analyzed by LC-QE HF. Labeling Efficiency: 99.5 % when 0.37 μg/μL mAb and 20 μg/μL N-Glycan tags are used to label 15 μg N-glycans released by glycoproteins. Thermal stability: N-Glycan labeling reagent is stable in anhydrous DMF at room temperature for 8 h; Labeled glycoamines are stable at room temperature for 24 h, and can withstand 320 centigrade of mass spectrometry ionization temperature. Chemical Instability: Unstable when concentrations of affinity reagents, SDS, Tris, DTT, amine, and mercaptans greater than 0.1 mM.

Specification

• Description: N-glycan label is used in ultra-fast labeling of glycans of PNGase F glycosidase-digested N-linked glycoproteins (including antibodies). Labeled glycan has highly sensitive fluorescence characteristics and ionization properties by mass spectrometry, allowing for quantitative analysis using LC-FLR and LC-MS. The unique chemical properties of this label provide higher fluorescence quantification and mass spectrometry response.
• Form: Lyophilized powder
• Molecular Mass: 427.1850 Da
• Applications: 4.5 mg N-Glycan labeling reagent can label five digested glycan samples.
• Storage: At -20 centigrade refrigerator as lyophilized powder.
• CAS No.: 61043-41-2
• Shelf life: 24 months at -20 centigrade.
• Usage Notes: Reference Procedure: 1. Add 10 μL desalted 1.5 μg/μL mAb samples into 0.6 mL low protein binding collection tube. 2. Add 10 μL 3 % BT Surfactant with 5 mM TCEP and 50 mM HEPES (pH 7.9) to the tube and mix well. 3. Heat at 95 centigrade for 3 min, then cool to the room temperature. 4. Add 10 μL 1.5 U/μL rPNGase F and mix well. 5. Incubated at 50 centigrade for 15-30 min, then cool to room temperature. 6. Add 10 μL 82 μg/μL Glycan Label, incubate at room temperature for 5 min. 7. Add 15 μL anhydrous DMF and 45 μL acetonitrile. Mix well. Labeled N-glycan samples can be used for LC-FLR and LC-MS analysis.
• Resuspension Buffer: Dissolve 4.5 mg N-Glycan label in 55 μL freshly prepared DMF solution to reach 82 μg/μL.