N'-Nicotinamide Methyltransferase (NNMT) Inhibitor Screening Assay Kit
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Cat.No. : | Kit-2215 |
Product Overview : | N′-Nicotinamide Methyltransferase (NNMT) (E.C. 2.1.1.1) catalyzes the N-methylation of nicotinamide, pyridines, and other analogues using S-adenosyl methionine (SAM) as the donor resulting in the production of 1-methylnicotinamide (MNA). NNMT plays a significant role in the regulation of metabolic pathways and is expressed at markedly high levels in several kinds of cancers, neurodegenerative diseases, obesity and diabetes, indicating it is a potential molecular target for therapy. NNMT inhibitor screening kit utilizes SAM as the methyl group donor and nicotinamide as the substrate. NNMT methylates nicotinamide generating S-adenosylhomocysteine (SAH) and 1-methylnicotinamide. The SAH is hydrolyzed by SAH hydrolase to form homocysteine, the free thiol group of which is detected using a Thiol Detecting Probe generating enhanced fluorescence signal that can be measured at Ex/Em = 392/482 nm. In the presence of an NNMT inhibitor, the enzymatic activity is inhibited resulting in decreased fluorescence. This assay kit is a simple, sensitive, and rapid tool to screen potential inhibitors of NNMT. |
Applications : | Screening/studying/characterizing potential inhibitors of NNMT. |
Storage : | -80°C |
Size : | 100 assays |
◆ Recombinant Protein
NNMT-5956H | Recombinant Human NNMT Protein, GST-tagged | +Inquiry |
NNMT-780H | Recombinant Human NNMT Protein, His-tagged | +Inquiry |
Nnmt-4449M | Recombinant Mouse Nnmt Protein, Myc/DDK-tagged | +Inquiry |
Nnmt-781M | Recombinant Mouse Nnmt Protein, His-tagged | +Inquiry |
NNMT-017H | Recombinant Human NNMT Protein, His/SUMO-tagged | +Inquiry |
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◆ Lysates
NNMT-3779HCL | Recombinant Human NNMT 293 Cell Lysate | +Inquiry |
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