N'-Nicotinamide Methyltransferase (NNMT) Inhibitor Screening Assay Kit
Cat.No. : | Kit-2215 |
Product Overview : | N′-Nicotinamide Methyltransferase (NNMT) (E.C. 2.1.1.1) catalyzes the N-methylation of nicotinamide, pyridines, and other analogues using S-adenosyl methionine (SAM) as the donor resulting in the production of 1-methylnicotinamide (MNA). NNMT plays a significant role in the regulation of metabolic pathways and is expressed at markedly high levels in several kinds of cancers, neurodegenerative diseases, obesity and diabetes, indicating it is a potential molecular target for therapy. NNMT inhibitor screening kit utilizes SAM as the methyl group donor and nicotinamide as the substrate. NNMT methylates nicotinamide generating S-adenosylhomocysteine (SAH) and 1-methylnicotinamide. The SAH is hydrolyzed by SAH hydrolase to form homocysteine, the free thiol group of which is detected using a Thiol Detecting Probe generating enhanced fluorescence signal that can be measured at Ex/Em = 392/482 nm. In the presence of an NNMT inhibitor, the enzymatic activity is inhibited resulting in decreased fluorescence. This assay kit is a simple, sensitive, and rapid tool to screen potential inhibitors of NNMT. |
- Specification
- Gene Information
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Applications : | Screening/studying/characterizing potential inhibitors of NNMT. |
Storage : | -80°C |
Size : | 100 assays |
Products Types
◆ Recombinant Protein | ||
NNMT-6125M | Recombinant Mouse NNMT Protein, His (Fc)-Avi-tagged | +Inquiry |
NNMT-185H | Recombinant Human NNMT Protein, MYC/DDK-tagged, C13 and N15-labeled | +Inquiry |
NNMT-034H | Recombinant Human NNMT Protein, His/SUMO-tagged | +Inquiry |
NNMT-5956H | Recombinant Human NNMT Protein, GST-tagged | +Inquiry |
Nnmt-781M | Recombinant Mouse Nnmt Protein, His-tagged | +Inquiry |
◆ Lysates | ||
NNMT-3779HCL | Recombinant Human NNMT 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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