|Product Overview:||This product is the Agarose bound Aleuria Aurantia Lectin and has sugar specificity against Fucose, Arabinose.|
|Description:||This lectin is a dimer of two identical subunits of about 36 kDa each. Unlike Ulex europaeus and Lotus tetragonolobus lectins which prefer (α -1,2) linked fucose residues, Aleuria aurantia lectin binds preferentially to fucose linked (α -1,6) to N-acetylglucosamine or to fucose linked (α -1,3) to N-acetyllactosamine related structures. AAL also reversibly binds fucose attached to nucleic acids|
|Form:||10 mM HEPES, pH 7.5, 0.15 M NaCl, 10 mM fucose, 0.08% sodium azide|
|Bio-activity:||Binding Capacity: >4 mg of fucosyl glycoprotein/ml of gel|
|Molecular Mass:||72 kDa|
|Applications:||Glycobiology, Affinity Chromatography|
|Usage:||1, Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin.
2, Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution. Alternatively, 100 mM L-fucose in buffered saline can be used.
3, After use, wash the gel with several column volumes of buffered saline then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
|Storage:||Refrigerate at 4 centigrade. DO NOT FREEZE.|
|Concentration:||2 mg/ml of settled gel|