Native Erythrina Cristagalli Lectin Protein, Agarose bound


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Native Erythrina Cristagalli Lectin Protein, Agarose bound

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Cat.No. : Lectin-1772E
Product Overview : This product is the Agarose bound Erythrina Cristagalli Lectin and has sugar specificity to Galactose, N-Acetylgalactosamine, and Lactose.
Description : Erythrina cristagalli lectin consists of two different subunits of approximately 28 kDa and 26 kDa. The carbohydrate structure to which ECL binds is frequently found in membrane and serum glycoproteins of mammalian origin. Sialic acid substitution on this structure appears to prevent the lectin from binding. This specificity offers an opportunity to utilize agarose bound ECL to isolate or fractionate mammalian glycoproteins.
Source : Erythrina cristagalli
Species : Erythrina cristagalli
Form : 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM Ca2+, 20 mM lactose, 0.08% sodium azide
Bio-activity : Inhibiting/Eluting Sugar: 200 mM lactose
Molecular Mass : 54 kDa
Applications : Glycobiology, Affinity Chromatography
Usage : 1, Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin.
2, Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution. Alternatively, 0.2 M lactose can be used.
3, After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
Storage : Refrigerate. DO NOT FREEZE.
Concentration : Protein Concentration: 3.0 mg/ml of settled gel
Synonyms : Lectin; ECL; ECA
UniProt ID : P83410

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