|Product Overview :||This product is the Agarose bound Galanthus Nivalis Lectin and has sugar specificity against Mannose.|
|Description :||Galanthus nivalis lectin, unlike most mannose-specific lectins, is not a metalloprotein and does not require Ca2+ or Mn2+ for binding. Binding seems to be preferentially directed toward structures containing (α-1,3) mannose residues. Also in contrast to most mannose-binding lectins, GNL will not bind α-linked glucose. Reports indicate that this lectin binds rat and mouse IgM but not IgG. The only protein from human serum reported to bind to this lectin is α2-macroglobulin. GNL binds to many viral glycoproteins.|
|Source :||Galanthus nivalis|
|Species :||Galanthus nivalis|
|Form :||10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM Ca , 20 mM mannose, 0.08% sodium azide, 0.01 mM Mn|
|Bio-activity :||Inhibiting/Eluting Sugar: 100 mM - 200 mM α-methylmannoside|
|Molecular Mass :||50 kDa|
|Applications :||Glycobiology, Affinity Chromatography|
|Usage :||1, Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin.
2, Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution. Alternatively, 0.1 M α methyl mannoside can be used. For those glycoconjugates having a very high affinity for GNL, it may be necessary to lower the pH of the eluting sugar solution to pH 4.0 with acetic acid and increase the concentration of the α methyl mannoside to 0.5 M.
3, After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
|Storage :||Refrigerate. DO NOT FREEZE.|
|Concentration :||Protein Concentration: 3.0 mg/ml of settled gel|
|Synonyms :||Lectin; GNL|
|UniProt ID :||P30617|