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Native Horse gram Dolichos biflorus Lectin (DBA), HRP-Conjugated

Cat.No. : DBA-353H
Product Overview : Native Horse gram Dolichos biflorus Lectin (DBA) - HRP (Horseradish Peroxidase)
Blood Group Specificity: Non-specific
Preferred Sugar Specificity: α-N-Acetylgalactosamine
Inhibiting or Eluting Sugar: N-Acetylgalactosamine
Divalent Ions: Ca++, Mn++, Mg++, Zn++
Mitogenic Activity: No
Hazardous Shipping: Non-hazardous
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Description : Dolichos biflorus agglutinin (DBA) is isolated from horse gram and purified by affinity chromatography. DBA is a glycoprotein with a molecular weight of about 111,000 and consists of 4 subunits of equal size. This lectin has an isoelectric point between pH 4. 6 and pH 5. 5. DBA has been used as a general marker of developing renal collecting ducts, which can then be used to establish secretion status in blood group A individuals by hemagglutination inhibition and for detecting blood typing. DBA is over thousand times more active in agglutinating blood group A1 cells versus A2 cells, and is used to distinguish these blood types in routine serology applications, and has potential applications in examining tumors which affect the levels of blood group substances. Reconstitute with sterile buffer. Calcium, manganese, magnesium, and zinc ions are required for binding. Horseradish peroxidase (HRP) is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or release of light as a byproduct of the reaction. It is most commonly used for blotting, immunoassays and immunohistochemistry methods. Dolichos biflorus Lectin (DBA) is conjugated to HRP at an appropriate ratio to provide optimal staining characteristics.
Source : Horse gram
Species : Horse gram
Form : Liquid
Purity : High Purity Grade
Stability : Shelf Life: 1 year
Storage : At 2-8 centigrade.
Concentration : 5 mg/mL
Official Symbol : DBA
Synonyms : Dolichos biflorus Lectin; DBA
Official Symbol : DBA
Synonyms : Dolichos biflorus Lectin; DBA

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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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