Native Human Nucleosome

• Cat.No.: NUC-0003
• Product Overview: Native Human nucleosome, was expressed in HEK293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Nucleosomes prepared from mammalian HEK293 cells as a substrate for histone methyltransferase assay.
• Form: 20 mM Tris-HCl, pH 7.5, 3 mM DTT, 1 mM EDTA, and 10 mM Na butyrate and 20% glycerol.
• Applications: Substrate for histone methyltransferase assays. Ideal for screening small molecular inhibitors of histone methyltransferases for drug discovery and HTS applications.
• Usage: 1 ml for 50 reactions in a 96-well format.
• Notes: The concentration of this product is determined by Bradford and represents only the protein concentration.
• Storage: At least 6 months at –80°C.
• Shipping: -80°C
• Warning: Storing diluted protein is not recommended, if necessary, use a carrier protein (BSA 0.1% - 0.5%). Avoid freeze/thaw cycles.