Native Kidney bean Phaseolus vulgaris Lectin (PHA-E), TRITC-Conjugated

• Cat.No.: PHA-E-506K
• Product Overview: Native Kidney bean Phaseolus vulgaris Lectin (PHA-E) - TRITC (Rhodamine)
  Blood Group Specificity: Non-specific
  Preferred Sugar Specificity: Complex N-Glycans
  Inhibiting or Eluting Sugar: Bovine Thyroglobulin, Acetic Acid
  Divalent Ions: Ca++, Mn++
  Excitation: 545-555 nm
  Emission: 570-580 nm
  Mitogenic Activity: Yes
  Hazardous Shipping: Non-hazardous
  Fluorescence: Orange

Specification

• Species: Kidney bean
• Source: Kidney bean
• Description: Affinity purified Phaseolus vulgaris lectin (PHA-E) is tetrameric glycoprotein and responsible for the erythroagglutinating properties of the PHA fraction. It has a carbohydrate specificity towards oligosaccharides and elutes with bovine thyroglobulin or acetic acid. PHA-E will bind to both human erythrocytes and lymphocytes, with a specificity towards blood group A (-SA). There are five times more PHA-E receptors on normal human lymphocytes than there are on erythrocytes. PHA-E binds di-galactosylated and bisected N-glycan. This lectin is widely used as a biochemical tool for detecting bisecting GlcNAc- and Gal-bearing glycoproteins. Affinity purified Phaseolus vulgaris Lectin (PHA-E) is labeled with tetramethylrhodamine isothiocyanate (TRITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. TRITC is a bright orange or red-fluorescent dye with excitation ideally suited to the 532-nm laser line. TRITC labeled PHA-E can be used for cellular imaging applications.
• Form: Liquid
• Purity: High Purity Grade
• Stability: Shelf Life: 1 year
• Storage: At -20 centigrade.
• Concentration: 2 mg/mL

Gene Information

• Official Symbol: PHA-E
• Synonyms: Phaseolus vulgaris Lectin; PHA-E)