|Product Overview:||This product is the Agarose Bound Peanut Agglutinin (PNA) Lectin and has sugar specificity against Galactose.|
|Description:||Peanut agglutinin binds preferentially to the T-antigen, a galactosyl (β-1,3) N-acetylgalactosamine structure present in many glycoconjugates such as M and N blood groups, gangliosides, and many other soluble and membrane-associated glycoproteins and glycolipids. With certain exceptions, the receptor sequence for PNA is normally sialylated which prevents the lectin from binding to its receptor oligosaccharide (see Jacalin). Even sialic acid which is not bound directly to the receptor sugars may inhibit binding. The presence of calcium ions in diluents can enhance the binding of PNA to receptors, possibly by neutralizing the negative charges on sialic acid residues adjacent to the receptor sequence.|
|Form:||10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM Ca2+, 20 mM galactose, 0.08% sodium azide|
|Bio-activity:||Inhibiting/Eluting Sugar: 200 mM galactose or Glycoprotein Eluting Solution|
|Molecular Mass:||110 kDa|
|Applications:||Glycobiology, Affinity Chromatography|
|Usage:||1, Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin.
2, Use of buffers containing 0.1 mM Ca2+ and 0.01 mM Mn2+ is recommended.
3, Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution. Alternatively, 200 mM galactose in 10 mM HEPES-buffered saline, pH 7.5 can be used. For those glycoconjugates having very high affinity for PNA, it may be necessary to lower the pH of the eluting sugar solution to pH 3.0 with acetic acid.
|Storage:||Refrigerate. DO NOT FREEZE.|
|Concentration:||5 mg/ml of settled gel|