|Product Overview:||This product is the Biotinylated Phaseolus Vulgaris Leucoagglutinin (PHA-L) and has sugar specificity against galactose and complex structures.|
|Description:||Phaseolus vulgaris agglutinin is the name ascribed to a family of lectins, each of which consists of four subunits. There are two different types of subunits. One appears to be involved primarily in red cell agglutination and has been designated the “E” subunit (for erythroagglutinin). The other type is involved in lymphocyte agglutination and mitogenic activity and has been termed the “L” subunit (for leucoagglutinin). These subunits combine to produce five isolectins. PHA-L, with four "L" type subunits, does not agglutinate red cell but is a potent mitogen.|
|Bio-activity:||10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM Ca2+, 0.08% sodium azide, 0.01 mM Mn2+|
|Molecular Mass:||126 kDa|
|Applications:||Glycobiology, Affinity Chromatography|
|Usage:||1, Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution. Alternatively, 100 mM acetic acid or 50 mM glycine, pH 3.0 can be used.
2, After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
|Storage:||Refrigerate. DO NOT FREEZE.|
|Concentration:||3.0 mg/ml of settled gel|