|Product Overview:||This product is the Agarose bound Sambucus Nigra Lectin and has sugar specificity against Sialic Acid.|
|Description:||Sambucus nigra lectin, isolated from elderberry bark, binds preferentially to sialic acid attached to terminal galactose in α-2,6 and to a lesser degree, α-2,3 linkage. Binding is also inhibited to some extent by lactose or galactose. This lectin does not appear to bind sialic acid linked to N-acetylgalactosamine. SNA has been reported to inhibit cell-free protein synthesis.|
|Form:||10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM Ca2+, 0.08% sodium azide, 20 mM lactose|
|Bio-activity:||Carbohydrate-Binding Specificity: Sialic Acid|
|Molecular Mass:||140 kDa|
|Applications:||Glycobiology, Affinity Chromatography|
|Usage:||1, Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin.
2, Suggested conditions for eluting glycoconjugates: 0.5 M lactose in buffered saline followed by 0.5 M lactose in 0.2 M acetic acid.
|Storage:||Refrigerate. DO NOT FREEZE.|
|Concentration:||3.0 mg/ml of settled gel|
|Synonyms:||Lectin; SNA; EBL|