|Product Overview:||This product is the Agarose bound Vicia Villosa Lectin and has sugar specificity against N-Acetylgalactosamine.|
|Description:||This lectin is a family of tetrameric glycoproteins consisting of combinations of A and B subunits similar in structure to PHA and GSL I. The dominant isolectins in our preparations appear to be B subunit-rich. VVL recognizes preferentially α- or β-linked terminal N-acetylgalactosamine, especially a single α-N-acetylgalactosamine residue linked to serine or threonine in a polypeptide (the Tn antigen). Evidence suggests that this lectin also may require specific amino acid sequences at the receptor site of glycosylation. The disaccharide galactosyl (α-1,3) N-acetylgalactosamine is also a potent inhibitor of this lectin.|
|Form:||10 mM HEPES, pH 7.5, 0.15 M NaCl, 20 mM galactose 0.08% sodium azide|
|Bio-activity:||Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine|
|Molecular Mass:||102 kDa - 144 kDa|
|Applications:||Glycobiology, Affinity Chromatography|
|Usage:||1, Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin.
2, Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting solution. Alternatively, 200 mM N-acetylgalactosamine can be used. Elution can also be achieved with 100 mM sodium acetate, pH 3.0, 1 M galactose.
3, After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
|Storage:||Refrigerate. DO NOT FREEZE.|
|Concentration:||3.0 mg/ml of settled gel|
|Synonyms:||Lectin; VVL; VVA|