Active Native Vicia Villosa Lectin Protein, Agarose bound
Cat.No. : | Lectin-1855V |
Product Overview : | This product is the Agarose bound Vicia Villosa Lectin and has sugar specificity against N-Acetylgalactosamine. |
- Specification
- Gene Information
- Related Products
Description : | This lectin is a family of tetrameric glycoproteins consisting of combinations of A and B subunits similar in structure to PHA and GSL I. The dominant isolectins in our preparations appear to be B subunit-rich. VVL recognizes preferentially α- or β-linked terminal N-acetylgalactosamine, especially a single α-N-acetylgalactosamine residue linked to serine or threonine in a polypeptide (the Tn antigen). Evidence suggests that this lectin also may require specific amino acid sequences at the receptor site of glycosylation. The disaccharide galactosyl (α-1,3) N-acetylgalactosamine is also a potent inhibitor of this lectin. |
Source : | Vicia Villosa |
Species : | Vicia Villosa |
Form : | 10 mM HEPES, pH 7.5, 0.15 M NaCl, 20 mM galactose 0.08% sodium azide |
Bio-activity : | Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine |
Molecular Mass : | 102 kDa - 144 kDa |
Applications : | Glycobiology, Affinity Chromatography |
Usage : | 1, Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. 2, Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting solution. Alternatively, 200 mM N-acetylgalactosamine can be used. Elution can also be achieved with 100 mM sodium acetate, pH 3.0, 1 M galactose. 3, After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage. |
Storage : | Refrigerate. DO NOT FREEZE. |
Concentration : | 3.0 mg/ml of settled gel |
Synonyms : | Lectin; VVL; VVA |
UniProt ID : | P56625 |
Synonyms : | Lectin; VVL; VVA |
UniProt ID : | P56625 |
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