"Neprilysin" Related Products

Neprilysin Activity Assay Kit (Fluorometric)

Cat.No.: Kit-1049
Description: Neprilysin (NEP, EC 3.4.24.11), also known as neutral endopeptidase, enkephalinase, CD10, and common acute lymphoblastic leukemia antigen, is a zinc-containing transmembrane metalloproteinase. It is able to hydrolyze very important endogenous peptides, such as natriuretic atrial factor, enkephalins, substance P, bradykinin and amyloid β (Aβ) peptide. Thus, NEP is a potentially therapeutic target in important pathological conditions such as cardiovascular disease, prostate cancer, and Alzheimer’s disease. NEP has also been used as a biological marker of a type of child leukemia and the detection of NEP in endometrial stromal cells had been proposed as a helpful tool in diagnosis of endometriosis. NEP is currently a focus of major interest in cardiovascular and neurological research. Neprilysin Activity Kit utilizes the ability of an active NEP to cleave a synthetic substrate (Abz-based peptide) to release a free fluorophore. The released Abz can be easily quantified using a fluorescence microplate reader. The substrate is specific to NEP and can differentiate the NEP activity from Trypsin and other structurally similar zinc metalloproteinase in biological samples such as Angiotensin-Converting Enzymes (ACE1, ACE2), Endothelin Converting Enzymes (ECE1, ECE2). Our assay kit is simple, specific and can detect as low as 20 μU/mg of NEP activity.
Applications: NEP Activity Assay ishighly sensitive with a detection limit of approximately 20 μU/mg
Storage: -20°C
Shipping: Gel Pack
Size: 100 assays
Kit Components: NEP Assay Buffer; Neprilysin (Lyophilized); NEP Substrate (in DMSO); Abz-Standard (1 mM)
Target Species: Eukaryotes
Detection method: Fluorometric (Ex/Em = 330/430 nm)
Features & Benefits: Simple and reliable test NEP activity in biological samples;
High-throughput compatible;
Includes NEP positive control

Online Inquiry

Note: There will be extra charge for optional service!
Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information


Price does not include shipping and packaging costs.Request quote for final price.

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.