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NOS Activity Assay Kit

Cat.No. : Kit-0631
Product Overview : The NOS activity assay kit measures NOS activity by monitoring the conversion of radiolabeled arginine to citrulline. This assay is simple, sensitive, and specific for NOS activity and can be used with both crude and purified enzyme preparations. The kit includes sufficient materials and reagents for 50 total reactions. Radiolabeled arginine and NADPH are not included with the kit.For routine assays, radioactive arginine is added to protein extracts or purified NOS samples. After incubation, the reactions are stopped with a buffer containing ethylenediaminetetraacetic acid (EDTA), which chelates the calcium required by nNOS (neuronal NOS; NOS I) and eNOS (endothelial NOS; NOS III) and, consequently, inactivates the enzyme. In the case of iNOS (inducible NOS; NOS II), the low pH of the Stop Buffer, pH 5.5, stops the enzyme-catalyzed reaction. Equilibrated Resin, which binds to the arginine, is added to the sample reactions and the reactions are then pipetted into spin cups. The citrulline, being ionically neutral at pH 5.5, flows through the cups completely. NOS activity is then quantitated by counting the radioactivity in the eluate.
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Storage : Stability: 1 year;Storage: -80°C;This kit will perform as specified if stored as listed in the Materials Supplied and used before the expiration date indicated on the outside of the box.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (4)

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In which cells is NOS present? 03/31/2022

NOS are present in neurophagocytes, endothelial cells, macrophages.

Can NOS add tags other than his? 05/05/2021

Of course, we will provide you with the most suitable tags according to your needs.

NOS is closely related to the study of which diseases? 04/03/2021

NOS is associated with abnormal vascular endothelial function, cerebral infarction and other processes.

What is the effect of lipid-lowering drugs to NOS expression? 03/01/2021

Lipid-lowering drugs can increase the expression of NOS.

Customer Reviews (3)

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Reviews
02/18/2023

    The NOS proteins purchased from your company is suitable for ELISA.

    03/12/2022

      The technical expert will follow up on the progress of my experiments and provide constructive advice on the problems that arise in my experiments.

      12/01/2020

        Cheap price, high quality products, worth buying again.

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