PDI assay Kit


Home / Products / PDI assay Kit

PDI assay Kit

PDI Related Products

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

Cat.No. : Kit-0670
Size : 2x96 tests/50 tests
Description : The PDI assay kit provides a simple, homogenous assay for screening modulators of PDI enzymatic activity. This is accomplished by monitoring PDI-catalyzed reduction of insulin in the presence of Dithiothreitol (DTT), resulting in the formation of insulin aggregates which then bind avidly to the red-emitting fluorogenic ProteoStat PDI detection dye. Relative to analogous turbidimetric assays of PDI activity, the fluorescence-based assay provides a vastly improved assay signal window, improved lower detection limit, and superior Z"-score (>0.8). Intra-plate and inter-plate CVs using the assay are typically 3-5%. Human recombinant PDI enzyme (EC and the PDI inhibitor, bacitracin, are included in the kit, as well as all reagents necessary for monitoring changes in PDI activity. The ProteoStat PDI assay kit is capable of providing a quantitative readout of PDI enzymatic activity in a robust and high-throughput fashion and can be applied to identification of PDI inhibitors from chemical libraries.
Applications : Fluorescence microscopy. This kit provides a simple, homogenous assay for screening modulators of protein disulfide isomerase (PDI) enzymatic activity.
Storage : -80°C
Kit Components : ProteoStat PDI detection reagentPDI (human) (recombinant)InsulinBacitracin (inhibitor control)PBE bufferStop reagentDTTDeionized water

Online Inquiry

  • Note: There will be extra charge for optional service!
  • Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional Service: Optional requirements on this protein

Other Requirements:

Apply For A Coupon

$50 OFF Your First Purchase

Apply For a Coupon

Enter your email here to subscribe.

creative biomart inc.

Easy access to products and services you need from our library via powerful searching tools.

Follow Us

Copyright © 2007 – 2020 Creative BioMart. All Rights Reserved.