Phospholipase D Assay Kit

Cat.No.: Kit-0704
Product Overview: Phospholipase D Assay Kit is a quantitative colorimetric/fluorimetric phospholipase D activity determination.
Description: PHOSPHOLIPASE D (PLD) catalyses the hydrolysis of the phosphodiester bond of glycerophospholipids to generate phosphatidic acid and a free headgroup. Abnormalities in PLD expression have been associated with human cancers.
Applications: Direct Assays: phospholipase D in biological samples.Drug Discovery/Pharmacology: effects of drugs on phospholipase D metabolism.
Usage: For research use only (RUO)
Storage: Store all components at -20°C. Shelf life of six months after receipt.
Kit Components: Assay Buffer 10 mLDye Reagent 120 µLEnzyme Mix (Dried) 1 vialSubstrate 1.5 mLCalibrator 400 µL
Detection method: Colorimetric, Fluorometric
Compatible Sample Types: Biological Sample
Features & Benefits: Sensitive. Use 10 µL samples. Detection range: colorimetric assay 0.06 - 10 U/L, fluorimetric assay 0.04 - 1 U/L.Simple and High-throughput: the assay involves addition of a single working reagent and can be readily adapted to high-throughput assays for drug screening.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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