"Phospholipase D" Related Products

      Phospholipase D Assay Kit

      Cat.No. : Kit-2483
      Product Overview : Phospholipase D (PLD) catalyses the hydrolysis of the phosphodiester bond of glycerophospholipids to generate phosphatidic acid and a free headgroup. Abnormalities in PLD expression have been associated with human cancers. method provides a simple and high-throughput assay for measuring PLD activity. In this assay, PLD hydrolyzes phosphatidylcholine to choline which is determined using choline oxidase and a H2O2 specific dye. The optical density of the pink colored product at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the PLD activity in the sample.
      Storage : -20ºC
      Shipping : On Ice
      Size : 100 tests
      Detection method : OD570nm, or FL530/585nm
      Compatible Sample Types : Biological
      Features & Benefits : Sensitive. Use 10 μL samples. Detection range: colorimetric assay 0.06 - 10 U/L, fluorimetric assay 0.04 - 1 U/L.
      Simple and High-throughput: the assay involves addition of a single working reagent and can be readily adapted to high-throughput assays for drug screening.
      Assay time : 30 min
      Sensitivity : 0.04 U/L

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