Phospholipase D (PLD) Activity Colorimetric Assay Kit

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Phospholipase D (PLD) Activity Colorimetric Assay Kit

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Cat.No. : Kit-2294
Product Overview : Phospholipase D (PLD) is a key player in phospholipid metabolism. PLD hydrolyzes the phosphodiester bond of the glycerophospholipids, resulting in the production of phosphatidic acid and a free headgroup. PLD activity regulates the actin cytoskeleton, vesicle trafficking for secretion and endocytosis, and receptor signaling. Abnormalities in PLD activity and expression have been associated with Alzheimers disease, stroke, cancer and other brain disorders. Phospholipase D Activity Assay Kit provides a simple and sensitive method of measuring PLD activity using colorimetry (OD 570 nm). In this assay, PLD cleaves choline fromPhosphatidylcholine. The free choline is then oxidized by PLD enzyme mix to generate an intermediate which reacts with PLD Probe to generate color (OD 570 nm). This high-throughput adaptable assay kit can detect PLD activity as low as 1.0 mU/ml in a variety of samples.
Applications : Measurement of PLD activity in various tissues/cells
Analyzing intracellular signaling
Screening of PLD inhibitors
Storage : -20°C
Size : 100 assays
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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