Phospholipase D (PLD) Activity Colorimetric Assay Kit

Cat.No.: Kit-2294
Product Overview: Phospholipase D (PLD) is a key player in phospholipid metabolism. PLD hydrolyzes the phosphodiester bond of the glycerophospholipids, resulting in the production of phosphatidic acid and a free headgroup. PLD activity regulates the actin cytoskeleton, vesicle trafficking for secretion and endocytosis, and receptor signaling. Abnormalities in PLD activity and expression have been associated with Alzheimers disease, stroke, cancer and other brain disorders. Phospholipase D Activity Assay Kit provides a simple and sensitive method of measuring PLD activity using colorimetry (OD 570 nm). In this assay, PLD cleaves choline fromPhosphatidylcholine. The free choline is then oxidized by PLD enzyme mix to generate an intermediate which reacts with PLD Probe to generate color (OD 570 nm). This high-throughput adaptable assay kit can detect PLD activity as low as 1.0 mU/ml in a variety of samples.
Applications: Measurement of PLD activity in various tissues/cells
Analyzing intracellular signaling
Screening of PLD inhibitors
Storage: -20°C
Concentration: Gel Pack
Shipping: Gel Pack
Size: 100 assays
Kit Components: PLD Assay Buffer; PLD Probe (in DMSO); PLD Substrate; PLD Enzyme Mix (Lyophilized); Choline Standard (Lyophilized); PLD Positive Control (Lyophilized)
Detection method: Absorbance (OD 570 nm)
Features & Benefits: Simple, rapid & convenient;
The assay can detect phospholipase D (PLD) Activity less than 1 mU/ml in various sample types.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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