|Product Overview:||A sensitive and robust homogeneous PI3-K assay that does not require radioactive materials or secondary (detector) enzymes or antibodies. The kit is ideally suited for automated screening (HTS) and can be read on any fluorimeter.|
|Size:||1 kit/100 Tests|
|Usage:||The Calbiochem PI 3-K Assay Kit is a homogeneous assay designed to measure PI 3-K activity using purified enzyme preparations.|
|Storage:||Storage: +2°C to +8°C;Storage Conditions: Upon arrival store the entire contents of the kit at 4°C.Do not freeze: No.|
|Kit Components:||Assay Buffer 3: 1 vial, 10 ml, supplied as 50 mM MgCl₂, 25 mM HEPES, 0.05% sodium azide, pH 7.0Coordination Buffer: 1 vial, 600 µl, supplied as 1X, pH 9.5Sensor Stock: 1 vial, 45 µl, supplied in DMSO/methanol (1:1)Sensor Dilution Buffer: 1 vial, 10 ml, supplied as a MES/NaCl-based buffer, 0.05% sodium azideTrivalent Metal Ion: 1 vial, 10 µl, supplied as 50 mM in 0.5 N HClPost Reaction Buffer: 1 vial (lot-specific volume), supplied as NaCl-based buffer, 0.05% NaN₃|
|Compatible Sample Types:||Purified enzyme|
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