pNPP Phosphatase Assay Kit

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pNPP Phosphatase Assay Kit

pNPP Phosphatase Related Products

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Cat.No. : Kit-2458
Product Overview : p-nitrophenyl phosphate (pNPP) is a chromogenic substrate for most phosphatases such as alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases. The reaction yields para-nitrophenol, which becomes an intense yellow soluble product under alkaline conditions and can be conveniently measured at 405 nm on a spectrophotometer. This homogeneous "mix-and-measure" assay involves simply adding a single reagent to the phosphatase and measuring the product formation on an absorbance reader. The assay can be conveniently performed in cuvettes, tubes or multi-well plates at either room temperature or 37°C.
Storage : 4, -20ºC
Size : 500 tests

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