PPAR delta Transcription Factor Assay Kit
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|Product Overview :||PPAR delta Transcription Factor Assay Kit is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the peroxisome proliferator response element (PPRE) is immobilized onto the bottom of wells of a 96-well plate. PPARs contained in a nuclear extract, bind specifically to the PPRE. PPAR delta is detected by addition of specific primary antibody directed against PPAR delta. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. ab133~10^6 detects human PPAR delta. This assay does not cross-react with PPAR gamma 1 and PPAR gamma 2 or PPAR alpha.|
|Size :||96 tests|
|Applications :||Functional Studies more details|