"PDI" Related Products

        Protein Disulfide Isomerases (PDI) Inhibitor Screening Kit (Fluorometric)

        Cat.No. : Kit-2378
        Product Overview : Protein Disulfide Isomerases (PDI, EC: constitute a thio-disulfide oxidoreductase family. PDI is abundant in the lumen of endoplasmic reticulum (ER). PDI plays an essential role in catalyzing the rearrangement of S-S bonds in proteins and functions as a chaperon. Recent studies show that PDI activity is essential for cancer cell survival and proliferation, and targeting the PDI activity with its inhibitors abrogates survival responses to ER stress in cancer cells. Thus, identification and development of PDI inhibitors represents an important approach in cancer therapy. In PDI Inhibitor Screening Kit, the PDI converts Insulin into its reduced form in the presence of Dithiothreitol (DTT). This reduced insulin binds to a fluorescent probe to generate an intensely fluorescent product (Ex/Em = 440/490 nm). In the presence of a PDI inhibitor, the reaction is impeded/abolished, resulting in a decrease or total loss of fluorescence. This assay kit can be used to screen/study/characterize the potential inhibitors of Protein Disulfide Isomerase. The assay is simple, high-throughput adaptable and can be performed within 60 min.
        Applications : Screening/characterizing/studying potential inhibitors of Protein Disulfide Isomerase (PDI)
        Storage : -20°C
        Shipping : Gel Pack
        Size : 100 assays
        Kit Components : PDI Assay Buffer; PDI Substrate; PDI Probe (in DMSO) (20X); DTT (100X); PDI Enzyme; PDI Inhibitor Control (Iodoacetamide)
        Detection method : Fluorescence (Ex/Em = 440/490 nm)
        Features & Benefits : Simple and High throughput adaptable
        60 minutes short protocol
        Detect using a fluorometer or fluorescence microplate reader.

        Online Inquiry

        Note: There will be extra charge for optional service!

        Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

        Optional requirements on this protein    +Expand

        Price Inquiry

        Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.