Protein Synthesis Kit
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|Product Overview :||E. coli cell-free protein expression method is the coupled reaction of transcription and translation. The method use a mixture containing template DNA bearing a gene of interest (either expression vector or PCR product), E. coli cell extract and other required reagents such as amino acids and rNTPs. rNTPs, and T7 RNA polymerase synthesize mRNA from template DNA, and then ribosome and tRNA are required for the translation step.
Ni-NTA magnetic beads have many advantages over existing resins and are suited for purification of His-tagged recombinant protein. Ni-NTA groups coated on surface of the beads can interact with His-tag of expressed protein to make that protein to bind them on the bead. After washing out unbound proteins, the target proteins can be purified through elution process.