"PTPase" Related Products

Protein Tyrosine Phosphatase Activity Assay Kit (Fluorometric)

Cat.No.: Kit-2196
Product Overview: Protein tyrosine phosphatases (PTPase(s), EC 3.1.3.48) are a diverse family of enzymes that catalyze the removal of a phosphate moiety from protein phosphotyrosine residues. Phosphorylation and dephosphorylation of tyrosine residues is a common post-translational modification that serves as a regulatory switch for many enzymes, receptors and signal transduction pathways. PTPs can be classified as intracellular (non-receptor) or receptor-like based upon their cellular localization, however all classical PTPs share a common catalytic mechanism utilizing a nucleophilic cysteine residue. As key regulators of cellular signaling cascades, PTPs affect cell growth, differentiation, migration and metabolism. Dysfunction of certain PTPs has been linked to several human diseases. For example, the intracellular tyrosine phosphatase PTP1B is a negative regulator of the insulin signaling cascade and has also been demonstrated to be dramatically overexpressed in human breast, colon and ovarian cancers. PTPase Activity Assay Kit enables rapid measurement of PTPase activity, utilizing a fluorogenic protein phosphatase substrate that is converted into a highly fluorescent product (Ex/Em = 368/460 nm). A broad-spectrum PTPase inhibitor is provided for verification of specific activity in complex biological matrices, where serine/threonine phosphatases may contribute to substrate metabolism. Unlike ELISAs or malachite green-based approaches, the assay is homogeneous, continuous and does not require complicated sample processing or desalting to eliminate free phosphate. The assay is simple to perform, high-throughput adaptable and can detect a minimum of 0.1 mU PTPase activity.
Applications: Rapid assessment of PTPase activity in biological samples or recombinant PTPase preparations.
Storage: -20°C
Shipping: Gel Pack
Size: 100 assays
Kit Components: PTPase Assay Buffer; Disulfide Reducing Agent (DTT); Fluorescent Standard; PTPase Inhibitor (Suramin); PTPase Substrate; PTPase Positive Control
Target Species: Mammalian
Detection method: Fluorescence (368/460 nm)
Features & Benefits: Simple procedure; takes less than 1 hour;
Fast and convenient;
The kit is more sensitive than colorimetric assays using phosphate detection.

Online Inquiry

Note: There will be extra charge for optional service!
Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information


Price does not include shipping and packaging costs.Request quote for final price.

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.