Rabbit Monoclonal Antibody to Human Tumor-Associated Calcium Signal Transducer 2

Name: Rabbit Monoclonal Antibody to Human Tumor-Associated Calcium Signal Transducer 2
SKU: CAB11611RH

Species :	Human
Source :	Rabbit
Tag :	Non
Antigen Description :	Human TROP2, also referred to as tumor associated calcium signal transducer 2 (TACSTD2), GA733-1 or M1S1, is a cell surface glycoprotein highly expressed by human carcinomas. TROP2 encoding by an intronless gene was originally defined by the monoclonal antibody GA733, and is a member of a family of at least two type Imembrane proteins. The other known member is GA733-2, also called EpCAM and TROP1. It has been suggested by studies that the GA733-1 gene was formed by the retroposition of the GA733-2 gene via an mRNA intermediate. The cytoplasmic tail of TROP2 possesses potential serine and tyrosine phosphorylation sites and a conserved phosphatidyl-inositol binding consensus sequence. TROP2 transduces an intracellular calcium signal and acts as a cell surface receptor, meanwhile is involved in the regulation of cell- cell adhesion. Accordingly, as a widespread stimulator of human cancer growth and a unique marker and causal factor of metastatic cancer, TROP2 is a possible candidate for diagnosis and molecular target therapy. Mutations of this gene result in gelatinous drop-like corneal dystrophy, an autosomal recessive disorder characterized by severe corneal amyloidosis leading to blindness.
Specificity :	Human TROP2 / TACSTD2. No cross-reactivity in ELISA withHuman BCAM / CD239; Human VCAM1 / CD106; Human ICAM1 / CD54; Human CD146 / MCAM / MUC18; Human CD171 / NCAML1 / L1CAM; Human Cadherin-1 / E-cadherin / CDH1 / CD324
Immunogen :	Recombinant human TROP2 protein
Isotype :	Rabbit IgG
Clone :	A31
Applications :	Immunochemistry; Western blot; ELISA
Dilution :	IHC-P: 1-10 μg/mLWestern blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human TROP2 in WB. Using a DAB detection system, the detection limit for human TROP2 is approximately 0.5 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommendedDirect ELISA: This antibody can be used at 0.1- 0.2 μg/mL with the appropriate secondary reagents to detect human TROP2. The detection limit for human TROP2 is 0.00245 ng/well
Preparation :	This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human TROP2 extracellular domain
Format :	0.2 μm filtered solution in PBS with 5% trehalose
Storage :	This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Gene Name	TACSTD2 tumor-associated calcium signal transducer 2 [ Homo sapiens ]
Official Symbol	TACSTD2
Synonyms	TACSTD2; tumor-associated calcium signal transducer 2; M1S1; EGP 1; GA733 1; TROP2; epithelial glycoprotein-1; cell surface glycoprotein TROP2; cell surface glycoprotein Trop-2; pancreatic carcinoma marker protein GA7331; pancreatic carcinoma marker protein GA733-1; gastrointestinal tumor-associated antigen GA7331; membrane component chromosome 1 surface marker 1; membrane component, chromosome 1, surface marker 1; 40kD glycoprotein, identified by monoclonalGA733; EGP1; GP50; EGP-1; GA7331; GA733-1;
Gene ID	4070
mRNA Refseq	NM_002353
Protein Refseq	NP_002344
MIM	137290
UniProt ID	P09758
Chromosome Location	1p32
Function	receptor activity;