Ral Activation Kit
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|Product Overview :||Ral Activation Kit provides a rapid, cost-effective and reliable tool for the detection and semi-quantitative analysis of the cellular activation state of Ral GTPases. The kit exploits the selective interaction of the Ral-binding domain (RalBD) of the Ral-effector RIP1 (also known as RLIP or RalBP1) with the active, Ral-GTP conformation. Recombinant, GST-tagged RalBD is added to cell extracts to pull out Ral-GTP, which is consequently detected by Western blotting. The kit detects active, GTP-bound RalA and RalB. However, the antibody included in the kit allows detection of RalA only. The kit also includes recombinant RalA protein that, once preloaded with GDP or GMPpNHp (all included in the kit), can be used to "spike" the cell extracts thus serving as an internal control of signal specificity.|
|Size :||1 kit|
|Applications :||Functional Studies more details|