Recombinant 2019-nCoV N protein


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Recombinant 2019-nCoV N protein

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Cat.No. : N-127V
Product Overview : Recombinant 2019-nCoV N protein was expressed in E. coli and purified by Ni column.
Description : Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Source : E. coli
Species : 2019-nCov
Form : Liquid. PBS, pH 8.0.
Molecular Mass : 47.08 kDa
Storage : For short term storage, store at 4 centigrade. For long term storage, store at -20~-70 centigrade, avoiding freeze/thaw cycles.
Concentration : 1.0 mg/ml
SDS-PAGE of N-127V :

ELISA of N-127V :

ELISA plate was coated by N protein, 100 µl/cell, at various concentrations. The direct ELISA analysis was performed by loading 100 µL per well of a anti C-ter His tag HRP conjugated mAb at a concentration of 1:2000. The plate was incubated for 1 hours at 37 centigrade, then washed 5 time. Detection was performed using TMB substrate for 10 minutes at room temperature in the dark. The plate was stopped with 2M sulfuric acid. Absorbances were read on a spectrophotometer at 450 nm.

Gene Name : N
Official Symbol : N
Synonyms : GU280_gp10
Gene ID : 43740575
Protein Refseq : YP_009724397.2
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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