Recombinant 2019-nCoV N protein


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Recombinant 2019-nCoV N protein

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Cat.No. : N-127V
Product Overview : Recombinant 2019-nCoV N protein was expressed in E. coli and purified by Ni column.
Description : Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Source : E. coli
Species : 2019-nCov
Form : Liquid. PBS, pH 8.0.
Molecular Mass : 47.08 kDa
Storage : For short term storage, store at 4 centigrade. For long term storage, store at -20~-70 centigrade, avoiding freeze/thaw cycles.
Concentration : 1.0 mg/ml
SDS-PAGE of N-127V :

ELISA of N-127V :

ELISA plate was coated by N protein, 100 µl/cell, at various concentrations. The direct ELISA analysis was performed by loading 100 µL per well of a anti C-ter His tag HRP conjugated mAb at a concentration of 1:2000. The plate was incubated for 1 hours at 37 centigrade, then washed 5 time. Detection was performed using TMB substrate for 10 minutes at room temperature in the dark. The plate was stopped with 2M sulfuric acid. Absorbances were read on a spectrophotometer at 450 nm.

Gene Name : N
Official Symbol : N
Synonyms : GU280_gp10
Gene ID : 43740575
Protein Refseq : YP_009724397.2

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