Recombinant AAV-2 VP1 Protein, His tagged

• Cat.No.: VP1-100A
• Product Overview: Recombinant AAV-2 VP1 Protein with His tag was expressed in E. coli.

Specification

• Species: AAV-2
• Source: E.coli
• Tag: His
• Protein Length: 1-735 aa
• Description: Adeno-Associated Viruses (AAV) are small, non-enveloped viruses containing a small, linear single-stranded DNA (ssDNA) genome. Non-enveloped viruses do not have a lipid bilayer surrounding the viral particle and are only composed of a protein capsid and the viral genetic material inside. The AAV genome is ~4.7 kb long and consists of two open reading frames, Rep and Cap, flanked by two 145 bp inverted terminal repeats (ITRs). The Rep region encodes four non-structural replication proteins (Rep78, Rep68, Rep52, and Rep40), which are involved in genome replication and viral packaging. The Cap region encodes three structural capsid proteins or viral proteins (VP1, VP2 and VP3) that form the icosahedral capsid of the virus. The ITR sequences serve as origins of replication and also act as signals for packaging.
• AASequence: MGSSHHHHHHSSGLVPRGSHMAADGYLPDWLEDTLSEGIRQWWKLKPGPPPPKPAERHKDDSRGLVLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDRQLDSGDNPYLKYNHADAEFQERLKEDTSFGGNLGRAVFQAKKRVLEPLGLVEEPVKTAPGKKRPVEHSPVEPDSSSGTGKAGQQPARKRLNFGQTGDADSVPDPQPLGQPPAAPSGLGTNTMATGSGAPMADNNEGADGVGNSSGNWHCDSTWMGDRVITTSTRTWALPTYNNHLYKQISSQSGASNDNHYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTQNDGTTTIANNLTSTVQVFTDSEYQLPYVLGSAHQGCLPPFPADVFMVPQYGYLTLNNGSQAVGRSSFYCLEYFPSQMLRTGNNFTFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTNTPSGTTTQSRLQFSQAGASDIRDQSRNWLPGPCYRQQRVSKTSADNNNSEYSWTGATKYHLNGRDSLVNPGPAMASHKDDEEKFFPQSGVLIFGKQGSEKTNVDIEKVMITDEEEIRTTNPVATEQYGSVSTNLQRGNRQAATADVNTQGVLPGMVWQDRDVYLQGPIWAKIPHTDGHFHPSPLMGGFGLKHPPPQILIKNTPVPANPSTTFSAAKFASFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYNKSVNVDFTVDTNGVYSEPRPIGTRYLTRNL
• Molecular Mass: 84 kDa
• Purity: > 85% by SDS-PAGE
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: 50mM Tris pH8.0, 300mM NaCl, 10% glycerol
• Concentration: 1 mg/mL

Gene Information

• Official Symbol: VP1
• Synonyms: VP1; AAV-2 VP1