Recombinant Active Human NAE1/UBA3 Complex Protein, His/GST-tagged

• Cat.No.: NAE1-33H
• Product Overview: Recombinant human NAE1 (2-end) and full-length human UBA3 were expressed by baculovirus in Sf9 insect cells. NAE1 used an N-terminal His tag, the gene accession number is NM_003905. UBA3 used an N-terminal GST tag and the gene accession number is BC022853.

Specification

• Description: The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene.
• Source: Insect cell
• Species: Human
• Tag: His/GST
• Molecular Mass: NAE1: Approx 65 kDa
  UBA3: Approx 73 kDa
• Purity: > 70%
• Stability: One year at -70 centigrade from data of shipment.
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μL
• Storage Buffer: Recombinant protein complex is a combination of the folloeing 2 buffer systems, adjusted to optimize the NAE1 (phosphate buffer system) and UBA3 (Tris buffer system) proportions:
  50 mM sodium phosphate, pH 7.5, 300 mM NaCl, 150 mM imidazole, 0.25 mM DTT, 25% glycerol.
  50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 25% glycerol.
• Shipping: Dry ice

Gene Information

• Gene Name: NAE1 NEDD8 activating enzyme E1 subunit 1 [ Homo sapiens (human) ]
• Official Symbol: NAE1
• Synonyms: NAE1; NEDD8 activating enzyme E1 subunit 1; HPP1; ula-1; APPBP1; A-116A10.1; NEDD8-activating enzyme E1 regulatory subunit; APP-BP1; NEDD8-activating enzyme E1 subunit; amyloid beta precursor protein binding protein 1, 59kDa; amyloid beta precursor protein-binding protein 1, 59 kDa; amyloid beta precursor protein-binding protein 1, 59kD; amyloid protein-binding protein 1; proto-oncogene protein 1; protooncogene protein 1
• Gene ID: 8883
• mRNA Refseq: NM_003905
• Protein Refseq: NP_003896
• MIM: 603385
• UniProt ID: Q13564