Recombinant Alternaria alternata ENO protein
| Cat.No. : | ENO-563A |
| Product Overview : | Recombinant Alternaria alternata ENO was expressed in E. coli. |
- Specification
- Gene Information
- Related Products
| Description : | Alt a 2 (Alt a 6 according to the effective allergen nomenclature) is a lyophilized, recombinant protein with IgE-binding capacity. It was produced by heterologous expression in E. coli, purified by conventional biochemical methods. |
| Source : | E. coli |
| Species : | Alternaria alternata |
| Molecular Mass : | ~47,382 Dalton |
| Purity : | > 98% |
| Notes : | Enolase activity can be restored by dissolving the lyophilized protein to a concentration of 1 mg/ml in 4.5 M urea and dialyzing it over night against 10mM NaxHxPO4 pH 7.5, containing 2mM -Mercaptoethanole, and 2mM Mg2+.No enzymatic activity was observed after reconstitution in water/ -Mercaptoethanole. |
| Storage : | When stored at -20 centigrade the quality of the material will be maintained for several years. However, for short periods (max. 3 weeks) the lyophilized product may be kept at room temperature. After reconstitution store at -20 centigrade. Avoid repeated freezing/thawing. |
| Concentration : | 1 mg/ml |
| Reconstitution : | The material can be reconstituted with distilled water (or equivalent) or dilute buffers. Addition of 1-2 mM -Mercaptoethanol is recommended. If reconstituted to a concentration of 1 mg/ml, the phosphate buffer concentration will be 2 mM (pH 7,4). The product is soluble to more than 95% at a concentration of 2 mg/ml. |
| UniProt ID : | Q9HDT3 |
| UniProt ID : | Q9HDT3 |
Products Types
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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