Active Recombinant C.perfringens NA protein, His-tagged

• Cat.No.: NA-862C
• Product Overview: Active Recombinant C.perfringens NA protein(P10481.1) (Cys 2-Gln 382) was expressed in E.coli, with a N-terminal polyhistidine tag.

Specification

• Species: C.perfringens
• Source: E.coli
• Tag: His
• Protein Length: 2-382 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its ability to cleave a fluorogenic substrate, 2'-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid. The specific activity is 150,000 pmoles/min/μg (Activity of this protein is much better than world's famous brand) as measured under the described conditions.
• Molecular Mass: The recombinant C. Perfringens neuraminidase consisting of 388 amino acids and has a calculated molecular mass of 43.6 kDa. It migrates as an approximately 40 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.