Recombinant Cattle INS protein, His & GST-tagged

• Cat.No.: INS-560C

Specification

• Product overview: Recombinant Cattle INS (A5PJB2) aa. (Phe25~Ala54) fused with His & GST tag was produced in E. coli cells.
• Source: E. coli
• Species: Cattle
• Tag: His & GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 33.4kDa; Accurate Molecular Mass: 36kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Phe25~Ala54
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >92%
• Characteristic: Isoelectric point is 6.4.
• Applications: Cell culture; Activity Assays; In vivo assays.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: 20mM Tris, 150mM NaCl,pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: INS insulin [ Bos taurus (cattle) ]
• Official Symbol: INS
• Synonyms: INS; insulin; proinsulin
• Gene ID: 280829
• mRNA Refseq: NM_001185126.1
• Protein Refseq: NP_001172055.1
• UniProt ID: A5PJB2