"INS" Related Products

Recombinant Cattle INS protein, His & GST-tagged

Cat.No.: INS-560C
Product overview: Recombinant Cattle INS (A5PJB2) aa. (Phe25~Ala54) fused with His & GST tag was produced in E. coli cells.
Source: E. coli
Species: Cattle
Tag : His & GST
Form: Freeze-dried powder
Molecular Mass: Predicted Molecular Mass: 33.4kDa; Accurate Molecular Mass: 36kDa as determined by SDS-PAGE reducing conditions.
Protein length: Phe25~Ala54
Endotoxin: <1.0EU per 1ug (determined by the LAL method)
Purity: >92%
Characteristic: Isoelectric point is 6.4.
Applications: Cell culture; Activity Assays; In vivo assays.
Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Storage buffer: 20mM Tris, 150mM NaCl,pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% Trehalose and Proclin300.
Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex
Gene Name: INS insulin [ Bos taurus (cattle) ]
Official Symbol: INS
Synonyms: INS; insulin; proinsulin
Gene ID: 280829
mRNA Refseq: NM_001185126.1
Protein Refseq: NP_001172055.1
UniProt ID: A5PJB2

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