Active Recombinant Chicken IFNA1 protein, His-GST-tagged

Cat.No. : IFNA1-634C
Product Overview : Active Recombinant Chicken IFNA1 protein(P42165)(47~191 aa), fused with N-terminal His tag and GST tag, was expressed in E.coli.
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Species : Chicken
Source : E.coli
Tag : GST&His
Protein Length : 47-191 aa
Form : Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Bio-activity : Interferon-alpha (IFNa), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits. IFN-α has both anti-viral and immunomodulatory activities on target cells. To test the effect of IFNa on cell apoptosis, A549 cells were seeded into triplicate wells of 96-well plates at a density of 4,000 cells/well and allowed to attach overnight, then the medium was replaced with various concentrations of recombinant chicken IFNa diluted with 5% serum standard DMEM. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Apoptosis of A549 cells after incubation with IFNa for 72h observed by inverted microscope was shown. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant chicken IFNa for 72h. It was obvious that IFNa significantly decreased cell viability of A549 cells. The ED50 of recombinant chicken IFNa is 5.13 ug/ml.
Molecular Mass : 47.0kDa
Endotoxin : <1.0EU per 1μg (determined by the LAL method).
Purity : > 95% as determined by SDS-PAGE.
Storage : Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
Reconstitution : Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Not For Human Consumption!

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