Recombinant CHIKV(strain SL-CK1) E2 protein(Ser326-Gln666), His-tagged

• Cat.No.: E2-2427C
• Product Overview: Recombinant Chikungunya virus (CHIKV)(strain SL-CK1) E2/Envelope 2 (ADG95913.1) (Ser326-Gln666) was expressed in Insect Cells with a polyhistidine tag at the C-terminus.

Specification

• Species: CHIKV
• Source: Insect Cells
• Tag: His
• Protein Length: Ser326-Gln666
• Form: Lyophilized from sterile 20 mM Tris, pH 7.5, 500 mM NaCl, 10 % gly. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant Chikungunya virus (CHIKV)(strain SL-CK1) E2/Envelope 2 consists of 352 amino acids and predicts a molecular mass of 39.8 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.