Active Recombinant CMV gB protein, His tagged

• Cat.No.: gB-110C
• Product Overview: Active Recombinant CMV gB protein(Met 1-Lys 700&Arg 777-Val 907)(AAA45920.1, with furin cleavage site mutated from 'RTKR' to 'TTQT') was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: CMV
• Source: HEK293
• Tag: His
• Protein Length: 1-700 aa&777-907 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its ability to bind biotinylated Human CD209-Fc in functional ELISA.
• Molecular Mass: The recombinant human CMV glycoprotein B (transmembrane domain deletion) comprises 818 amino acids and has a predicted molecular mass of 93 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the recombinant CMV gB is approximately 130-140 kDa due to glycosylation.
• Endotoxin: < 1 EU/µg as determined by LAL test.
• Purity: > 70 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Official Symbol: gB