Recombinant COVID-19 3CLpro protein

• Cat.No.: 3CLpro-3534V
• Product Overview: Recombinant COVID-19 3CLpro protein(YP_009725301.1)(Ser1-Gln306) was expressed in E.coli.

Specification

• Species: Sars-CoV-2
• Source: E.coli
• Tag: Non
• Protein Length: Ser1-Gln306
• Tag: Non
• Form: Supplied as 0.22μm filtered solution in 25mM HEPES, 2.5mM DTT, 10% glycerol (pH 7.5).
• Bio-activity: Measured by its ability to cleave a peptide substrate, MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2.The specific activity is > 5 pmol/min/μg.
• Molecular Mass: The protein has a predicted MW of 33.8 kDa same as Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Valid for 12 months from date of receipt when stored at -80°C; Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.