Recombinant COVID-19 3CLpro protein, His-tagged

• Cat.No.: 3CLpro-82V
• Product Overview: Recombinant COVID-19 NSP8 protein (Ser3264-Gln3569) was fused to His-tag at the N-terminus and expressed in Baculovirus-Insect Cells.

Specification

• Description: 3C-like protease (3CLpro) is the main protease of Humann Coronavirus. 3C-like protease (3CLpro) is a key enzyme, as it cleaves several sites to produce non-structural proteins that are essential for genome replication and Coronavirus virion production, such as an RNA-dependent RNA polymerase, a helicase, ribonucleases and 3CLpro itself, from two types of polyproteins (pp1a and pp1ab). SARS-CoV 3CLpro exists as a homodimer and each protomer has an active site.
• Source: Baculovirus-Insect Cells
• Species: COVID-19
• Tag: His
• Predicted N Terminal: Met
• Form: Lyophilized from sterile PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80.
• Molecular Mass: The recombinant COVID-19 3CL Protease consists of 332 amino acids and predicts a molecular mass of 37.12 kDa.
• Protein length: Ser3264-Gln3569
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95% as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -20 centigrade to -80 centigrade.
• Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/ml. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
  Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Gene Name: ORF1ab
• Official Symbol: ORF1ab
• Synonyms: ORF1a polyprotein
• Gene ID: 43740578
• Protein Refseq: YP_009725295.1
• UniProt ID: P0DTD1