Recombinant COVID-19 (B.1.1.529 sublineage BA.2) Spike S1 protein(Met1-Arg685), His-tagged
Cat.No. : | Spike-1273V |
Product Overview : | Recombinant SARS-CoV-2 Spike S1 (YP_009724390.1, with mutation T19I, L24S, del25-27, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H) (Met1-Arg685) was expressed in HEK293 with a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant B.1.1.529 sublineage BA.2). |
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Source : | HEK293 |
Species : | COVID-19 |
Tag : | His |
Protein length : | Met1-Arg685 |
Form : | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | Immobilized ACE2 Protein, Human, Recombinant (mFc Tag) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (B.1.1.529 sublineage BA.2) Spike S1 Protein (His Tag), the EC50 is 60-180ng/mL. |
Molecular Mass : | The recombinant SARS-CoV-2 Spike S1 consists of 678 amino acids and predicts a molecular mass of 76.39 kDa. It migrates as an approximately 112.68 kDa band in SDS-PAGE under reducing conditions. |
Endotoxin : | < 1.0 EU per μg protein as determined by the LAL method. |
Purity : | > 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC. |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ] |
Official Symbol : | Spike |
Synonyms : | Spike; S1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1 |
Gene ID : | 43740568 |
Protein Refseq : | YP_009724390.1 |
UniProt ID : | P0DTC2 |
Products Types
◆ Lysates | ||
Spike-1741HCL | Recombinant Human coronavirus Spike cell lysate | +Inquiry |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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