Recombinant COVID-19 (B.1.640.2) Spike S1+S2 trimer protein(Met1-Gln1208), His-tagged

• Cat.No.: Spike-1232V
• Product Overview: Recombinant SARS-CoV-2 (B.1.640.2) Spike S1+S2 (YP_009724390.1, with mutations P9L, E96Q, 136-144del, R190S, D215H, R346S, N394S, Y449N, E484K, F490S, N501Y, D614G, P681H, T859N, F817P, A892P, A899P, A942P, K986P, V987P and furin cleavage site mutants) was expressed in HEK293 with bacteriophage T4 fibritin and a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant B.1.640.2).

Specification

• Source: HEK293
• Species: COVID-19
• Tag: His
• Protein length: Met1-Gln1208
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized ACE2 Protein, Human, Recombinant (mFc Tag) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (B.1.640.2) Spike S1+S2 trimer Protein (ECD, His Tag), the EC50 is 4-15ng/mL.
• Molecular Mass: The recombinant SARS-CoV-2 (B.1.640.2) Spike S1+S2 consists of 1222 amino acids and predicts a molecular mass of 135.30 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ]
• Official Symbol: Spike
• Synonyms: Spike; S1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1
• Gene ID: 43740568
• Protein Refseq: YP_009724390.1
• UniProt ID: P0DTC2