Recombinant COVID-19 Full Length Spike Protein(Val16-Pro1213, R683A, R685A), His-tagged, active trimer (MALS verified)

• Cat.No.: Spike-208V
• Product Overview: Recombinant COVID-19 full length active trimer Spike protein(R683A, R685A)(MALS verified) was fused to His-tag at the C-terminus and expressed in HEK293.

Specification

• Description: Protein S (PROS1) is glycoprotein and expressed in many cell types supporting its reported involvement in multiple biological processes that include coagulation, apoptosis, cancer development and progression, and the innate immune response. Known receptors bind S1 are ACE2, DPP4, CEACAM etc.. It's been reported that COVID-19 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
• Source: HEK293
• Species: COVID-19
• Tag: His
• Predicted N Terminal: Val16
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Molecular Mass: The protein has a calculated MW of 138.0 kDa. The protein migrates as 160-230 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Val16-Pro1213
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publication:
  Single-dose intranasal vaccination elicits systemic and mucosal immunity against SARS-CoV-2 (2021)
  Interfacial Water in the SARS Spike Protein: Investigating the Interaction with Human ACE2 Receptor and In Vitro Uptake in A549 Cells (2022)
  Determining Site-Specific Glycan Profiles of Recombinant SARS- CoV‐2 Spike Proteins from Multiple Sources (2023)

Gene Information

• Gene Name: S
• Official Symbol: S
• Synonyms: GU280_gp02
• Gene ID: 43740568
• Protein Refseq: YP_009724390.1